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包涵体蛋白提取试剂盒

包涵体蛋白提取试剂盒

产品型号:
TS686
产品规格:
10 g bacteria
产品类型:
蛋白提取
产品品牌:
Toscience
价      格:
600.00
电话: 021-60880534    在线留言
General Description
The expression of foreign proteins at high levels in E. coli often results in the formation of inclusion bodies composed of insoluble aggregates of the expressed protein. The inclusion bodies are recovered from bacterial lysates by centrifugation and are washed with Triton X-100 and EDTA to remove as much bacterial protein as possible from the aggregated foreign protein. To obtain soluble protein, the washed inclusion bodies are dissolved in denaturing agents and the released protein is then refolded by gradual removal of the denaturing reagents by dilution or dialysis. The kit has been used for extraction of many proteins. However, each protein may require a slightly different procedure, which must be determined empirically.
    Toscience®是上海通善生物科技有限公司(Shanghai Toscience Biotechnology Co., Ltd.)的自主产品品牌,通善生物是一家生产、研发和销售生物化学实验室全套用品(包括:生物、化学化工试剂,实验耗材,仪器设备等)的集约化供应商。

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    Toscience®用于通善生物自主研发生产的细胞因子、不锈钢细胞筛、抗体、生化试剂等产品。
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