Recombinant Murine IL-2

Source: Escherichia coli. 

Molecular Weight: Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids. 

Quantity: 5µg/20µg/1000µg 

AA Sequence: APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ 

Purity: > 95 % by SDS-PAGE and HPLC analyses. 

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg. 

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. 

Formulation: Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4. 

Endotoxin: Less than 1EU/µg of rMuIL-2 as determined by LAL method. 

Reconstitution: 

 

 

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the 

bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a 

concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and 

stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 

Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 

Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further 

evaluation purposes. NOT FOR HUMAN USE.