Recombinant Human IFN-γ

Molecular Weight: Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids. 

Quantity: 20µg/100µg/1000µg 

AA Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ 

Purity: >98% by SDS-PAGE and HPLC analyses. 

Biological Activity: Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 2.0 × 107 IU/mg. 

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. 

Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. 

Endotoxin: Less than 1EU/µg of rHuIFN-γ as determined by LAL method. 

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and 

stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. 

Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For 

maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to 

-70°C. Avoid repeated freeze/thaw cycles. 

Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further 

evaluation purposes. NOT FOR HUMAN USE. 

Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by Tlymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory 

and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation.