Recombinant Human PDGF-BB
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Source: Escherichia coli.
Molecular Weight: Approximately 24.8 kDa, a disulfide-linked homodimeric protein containing two 110 amino acid
residues polypeptide (B chain).
Quantity: 2µg/10µg/1000µg
AA Sequence: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ
CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Purity: > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation
assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3
× 105
IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2 m filtered concentrated solution in PBS, pH 7.4.
Endotoxin: Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to
-70 °C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE.
Human Platelet-derived Growth Factor-BB
Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch
and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three
subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia,
embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is
mediated by two receptors (PDGFR-α and PDGFR-β).
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Toscience®用于通善生物自主研发生产的细胞因子、不锈钢细胞筛、抗体、生化试剂等产品。
上海通善生物科技有限公司产品种类涉及分子生物学、细胞生物学、生物化学、生态学、劳防用品及食品检验等诸多领域的试剂、消耗产品和设备等，为科研单位、高校、制药企业等提供全方位的一站式实验解决方案。
其中美国透析实验用品、Millipore超滤管、韩国SPL细胞培养耗材、以色列BI细胞培养基、BD细胞培养产品、细胞分离液、微生物干粉培养基、OMEGA试剂盒、Abcam抗体、Santa Cruz抗体、R&D产品、pierce试剂盒、sigma试剂、Fisher蓝盖瓶、NUNC耗材、corning耗材、Axygen试剂盒和耗材是我们的优势产品。
Toscience®用于通善生物自主研发生产的细胞因子、不锈钢细胞筛、抗体、生化试剂等产品。