Recombinant Human VEGF165
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Source: Escherichia coli.
Molecular Weight: Recombinant human VEGF165 is a 38.2 kDa disulfide-linked homodimeric protein consisting of two
165 amino acid polypeptide chains.
Quantity: 2µg/10µg/50µg/100µg/1000µg
AA Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC
CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC
SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Purity: >95% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
assay using human umbilical vein endothelial cells(HUVEC) is less than 8 ng/ml, corresponding to a
specific activity of ＞ 1.3 × 105
IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin: Less than 1EU/μg of rHuVEGF165 as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
-70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE.
Human Vascular Endothelial Growth Factor
Vascular Endothelial Growth Factor is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of
endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a
prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor
metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1),
KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
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Toscience®用于通善生物自主研发生产的细胞因子、不锈钢细胞筛、抗体、生化试剂等产品。
上海通善生物科技有限公司产品种类涉及分子生物学、细胞生物学、生物化学、生态学、劳防用品及食品检验等诸多领域的试剂、消耗产品和设备等，为科研单位、高校、制药企业等提供全方位的一站式实验解决方案。
其中美国透析实验用品、Millipore超滤管、韩国SPL细胞培养耗材、以色列BI细胞培养基、BD细胞培养产品、细胞分离液、微生物干粉培养基、OMEGA试剂盒、Abcam抗体、Santa Cruz抗体、R&D产品、pierce试剂盒、sigma试剂、Fisher蓝盖瓶、NUNC耗材、corning耗材、Axygen试剂盒和耗材是我们的优势产品。
Toscience®用于通善生物自主研发生产的细胞因子、不锈钢细胞筛、抗体、生化试剂等产品。