Recombinant Human LR3 IGF-1 （Media Grade）

Molecular Weight: Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids. 

Quantity: 1mg/5mg/20mg/50mg 

AA Sequence: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC 

CFRSCDLRRL EMYCAPLKPA KSA 

Purity: 90-95 % by SDS-PAGE and HPLC analyses. 

Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. 

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. 

Formulation: Lyophilized from a 0.2 m filtered concentrated solution in 20 mM PB, pH 7.2. 

Endotoxin: Less than 5 EU/100 g of rHuLR3 IGF-1 as determined by LAL method. 

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and 

stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 

Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. 

Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further 

evaluation purposes. NOT FOR HUMAN USE. 

 

 

IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the 

downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3 IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.