
凝血酶,来源于牛血浆
凝血酶 来源于牛血浆
Thrombin from bovine plasma
lyophilized powder, 40-300 NIH units/mg protein (biuret)
产品描述
Frequently Asked Questions
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Analysis Note
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆(由血浆与等量生理盐水混合制得)作为底物,
Biochem/physiol Actions
丝氨酸蛋白酶,选择性断开纤维蛋白原中的精氨酸-甘氨
General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Lyophilized powder containing sucrose, sodium chloride and Tris.
Other Notes
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Preparation Note
Activated with bovine brain thromboplastin.
Physical form
Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock. 1