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BioVision公司透析管

2015年9月10日
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一、蛋白透析存在的问题
在处理蛋白和核酸过程中,往往需要去除可能会影响后续实验的小分子物质如还原剂(DTT、β-巯基乙醇、尿素),未反应的交联或标记试剂(如sulfo-SMCC、生物素)和防腐剂(如叠氮钠、硫柳汞)。透析和电洗脱等方法长期以来一直被用于纯化蛋白和核酸。然而这些传统方法耗费大量时间、造成样本损失和污染、样本浓度被大大稀释而往往需要进一步的浓缩。
 
BioVision的DiaEasyTM Dialyzer tubes为您解除上述困扰,这些透析管具有从1 kDa, 3.5 KDa, 6 – 8 KDa, 12 – 14 kDa, 25 KDa到50 kDa不同的分子量截留,样本体积可从10μl至20ml。这些套装包含了浮筏和/或支撑托盘,便于将管子放置在交换液和电洗脱液中。DiaEasyTM Dialyzer tubes和电洗脱附件组合可用于从非变性和变性聚丙烯酰胺凝胶中分离蛋白、蛋白-蛋白或蛋白-DNA复合物,从聚丙烯酰胺凝胶和琼脂糖凝胶中分离寡核苷酸、RNA和DNA。DiaEasyTM Dialyzer tubes管壁非常洁净,不含硫黄和重金属,经EDTA处理使回收的蛋白和核酸适合于分子生物学实验。DiaEasyTM Dialyzer tubes可快速安全轻松地加载和回收样本。
 
具有6种体积的管子进行透析或更换缓冲液: 10-250 µl, 50-800 µl, 0.1-3 ml, 10 ml, 15 ml and 20 ml.
高通量透析
制备样本用于MALDI-MS
样本浓缩
大规模蛋白透析如抗体和重组蛋白纯化
透析的多肽可短至10个氨基酸
病毒颗粒纯化
去除污染的小分子
组织培养物纯化
复合物形成研究(蛋白-蛋白、蛋白-DNA、蛋白-RNA)
对样本溶液、蛋白提取物或细胞提取物进行pH值调节或更换缓冲液
二维凝胶斑点分离 
从聚丙烯酰胺、琼脂糖或其他凝胶基质中分离蛋白、RNA、DNA或寡核苷酸(>20nt)
 
市场上最方便、最有效、客户友好的透析套装
一步有效的透析节省时间,无需其他设备
样本一次通过管子,避免反复的稀释和浓缩
一次过管减少了蛋白聚集和沉淀的风险,确保其高活性
洗脱时间只要10 - 150min
这些套装与HTP兼容
详细产品信息请登录(http://www.toscience.net/product.aspx?keyword=DiaEasy%E9%80%8F%E6%9E%90
官网介绍如下:

Dialysis Tubes

While working with proteins and nucleic acids, it is very often necessary to eliminate small molecular weight substances such as reducing agents [dithiothreitol (DTT), 2-mercaptoethanol (BME), urea], un-reacted crosslinking or labeling reagents (sulfo-SMCC, biotin) and preservatives (sodium azide, thimerosal) that might interfere in subsequent steps of the experimental protocol. Methods such as dialysis and electro-elution (for extraction of nucleic acids from gels) have long been used to purify proteins and nucleic acids. However, these traditional methods are riddled with numerous problems which may lead to tremendous time consumption, sample loss, contamination and high sample dilutions requiring further concentration.

BioVision's DiaEasy™ Dialyzer tubes product line offers a solution to most of these issues. Our Dialysis tubes come in easy to use sets of 10, 25 and 100 tubes each, catering to different molecular weight cut-offs of 1 kDa, 3.5 kDa, 6-8 kDa, 12-14 kDa, 25 kDa and 50 kDa for different sample volumes ranging from 10 µl to 20 ml. These sets also contain floating racks and/or supporting trays for convenient handling of the tubes in the exchange buffer and electro-elution. Thecombination of DiaEasy™ Dialyzer tubes and the electro-elution accessories provides a unique tool for extraction of any protein, protein-protein, or protein DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels, and for extraction of oligonucleotides, RNA, and DNA from both polyacrylamide and agarose gels. The DiaEasy™ tube's membranes are ultra-clean, sulfur and heavy metal free and EDTA treated which makes the harvested proteins and nucleic acids suitable for molecular biology experiments. These DiaEasy™ tubes allow rapid, secure and simple loading and recovery. They are very high performance dialysis tubes and are the most convenient, user friendly dialysis system on the market

Some applications of these tubes are:
  • Dialysis or buffer exchange of six different samples volumes: 10-250 µl, 50-800 µl, 0.1-3 ml, 10 ml, 15 ml and 20 ml.
  • High throughput dialysis (using a single beaker with several DiaEasy™ tubes on one floating pad).
  • Preparation of protein samples for MALDI-MS.
  • Samples concentration.
  • Large-scale protein dialysis such as antibodies and recombinant protein purification.
  • Peptide dialysis as small as 10 amino acids.
  • Virus-particle purification.
  • Removal of contaminating micro molecules.
  • Tissue culture extracts purification.
  • Removal of salts, surfactants, solvents and detergents.
  • Complex formation studies (protein-protein, protein-DNA and protein-RNA).
  • pH and buffer adjustment of sample solution, protein extract or cell extract.
  • 2D gel dots extraction.
  • Extraction of proteins, RNA, DNA or oligonucleotides (>20 nt) from polyacrylamide, agarose or any gel matrix in any running buffer.
Some advantages of using these DiaEasy™ Dialyzer tubes are:
  • Most convenient, efficient and user-friendly dialysis sets on the market.
  • Single step efficient dialysis saves time and eliminates the need for additional equipment.
  • Samples pass through the tubes once, eliminating repeated dilutions and concentrations.
  • Single step decreases the risk of potential protein aggregation and precipitation and ensures high activity retention.
  • Elution times are as low as 10 minutes and range upto 150 minutes based on multiple criteria including the sample content, size and volume.
  • These sets are HTP compatible.
Typical recovery Rates:

Dialysis of samples≥98%
Electroelution of DNA or RNA from Agarose gel≥90%
Electroelution of DNA or RNA from polyacrylamide gel≥90%
Electroelution of Protein from SDS-PAGE gel≥70%

In summary, BioVision offers a comprehensive line of DiaEasy™ Dialyzer tubes ideal for single step, efficient dialysis and electroelution of proteins, protein-DNA complexes, oligonucleotides, DNA, and RNA from polyacrylamide and agarose gels, compatible with multiple downstream applications like MALDI-MS, HPLC and numerous functional assays.
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